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. 2020 Dec 9;7:616768. doi: 10.3389/fmolb.2020.616768

FIGURE 2.

FIGURE 2

MIR497HG suppresses BCa cell growth, migration, and invasion in vitro. (A) The schematic of CRISPR/Cas13d system. (B) Knockdown of MIR497HG with Cas13d compared with corresponding RNAi. (C) The CCK-8 assays showed that silencing of MIR497HG promotes BCa cell proliferation in 5637 cell line. (D) Clonogenic assay was performed to measure the capacity of foci formation in 5637 cells stably expressing indicated plasmids. (E) Wound healing assay of 5637 cells stably expressing indicated plasmids. (F) Representative images of Transwell invasion assay for MIR497HG-silencing 5637 cells. Cell number was counted in six randomly captured pictures. Invasive ability was normalized to control. Data are shown as mean ± SD. n = 3 for technical replicates. *P < 0.05, **P < 0.01, and ***P < 0.001. P values are calculated by Student t test.