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. 2020 Dec 9;7:616768. doi: 10.3389/fmolb.2020.616768

FIGURE 4.

FIGURE 4

MiR-497 and miR-195 directly suppressed multiple key components in Hippo/Yap and TGF-β signaling. (A) The Kyoto Encyclopedia of Genes and Genomes analysis of miR-497 and miR-195 target genes. (B) Predicted miR-497 and miR-195 binding conversed (red) and poorly conversed (blue) sites in 3′ UTRs of Birc5, Yap1, CCND1, and Smad3. CDS, coding sequence. (C) Luciferase reporter assay of candidates predicted to be regulated by miR-497 or miR-195. Renilla luciferase activity was normalized to firefly activity. n = 3 independent experiments. *P < 0.01 and **P < 0.01 by Student t test. (D) Western blot analysis of Birc5, cyclin D1, Smad3, and Yap1 in the indicated cells. Gapdh served as the loading control. Representative of three independent experiments.