Figure 4.

IRF5 siRNA down-regulated the increased expression of IRF5 induced by LPS. Mice were treated with ICV injection of IRF5 siRNA or scramble RNA, followed by ICV injection of LPS 24 h later. Mice were sacrificed 4 h later and the brain tissues were collected. (A) The expression levels of IRF5 in the ipsilateral hemispheres were determined by Western blot. (B) The expression levels of IRF5 in the ipsilateral and contralateral hemispheres were determined by Western blot. (C) Densitometric quantification of the blots in (A, B) after being normalized with the GAPDH levels. n = 4 in each group, *P < 0.05, **P < 0.01. Values are the mean ± SEM.