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. 2020 Dec 9;11:593368. doi: 10.3389/fimmu.2020.593368

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Copyright © 2020 Yu, Wu, Li, Deng, Liu, Zhang, He, Zhao and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bixin reduces the proportion of Th1 and Th17 cell subsets. Subsets of Th1 and Th17 cells in CD4+ gate were analyzed by intracellular staining for IFN-γ and IL-17, respectively, in MNCs from the spleen (A) and CNS (B). (C, D). mRNA expression of the inflammatory cytokines IFN-γ and IL-17 were quantitated by qRT-PCR (E–F). IFN-γ and IL-17 levels in serum were measured by ELISA. Data shown in graphs represents the means ± SD (n = 5). **P < 0.01, vs. control group; ^##P < 0.01, vs. EAE group.