FIGURE 4.

(A) Neuronal, astrocytic, microglial, and oligodendrocytic known markers’ expression in the Dumitriu samples. T-tests were used to compare gene expression mean differences between diseased (PD) and non-diseased (Control) samples. (B) Estimates of the composition of Dumitriu samples in each main cell type based on the human cell type gene expression signature. Wilcoxon signed-rank tests were used to compare differences in proportions between diseased (PD) and non-diseased (Control) samples. (C) Sample factorial map (upper plot) of components 1 (PC1) and 2 (PC2) of Principal Component Analysis (PCA) of the gene expression in Dumitriu samples, and their neuronal proportion related to PC1 loadings (lower plot). Indicated in the respective axes’ labels are the percentages of data variance explained by PC1 and PC2. Kolmogorov-Smirnov tests were used to compare the distributions of PC1 and PC2 loadings between PD and Control samples, illustrated by the smoothed histograms along the respective axes of the PCA plot. In the lower plots, the colored solid lines represent the linear regressions between neuronal proportions and PC1 loadings for PD and Control Samples. The respective Pearson’s correlation coefficients (r) and associated significance (p) are also indicated. Legend: ns: non-significant, ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤0.01, *p ≤0.05.