Figure 3.
Reproducible Gene Expression Changes during Spaceflight
(A) Overlap of differentially expressed genes (FDR ≤10%) across each of the three spaceflight studies. Red/blue shading denotes significance of the corresponding overlap for upregulated (UR) and downregulated (DR) genes, respectively.
(B) Non-redundant, enriched Gene Ontology Biological Process terms for common UR/DR genes across microgravity studies (defined by FDR ≤10% and |LFC| > 0). Number of genes enriched in a given term are provided within associated boxes of the heatmap. Darker shading denotes greater significance.
(C) Predicted transcription factors of UR and DR gene sets.
(D) Quantity (in boxes) and significance (green shading scale) of enriched protein-protein interactions among UR and DR gene lists, across a range of interaction “confidence” cutoffs.
(E) Protein interactions (confidence >0.15) for UR and DR genes with a hub score >0.6. Larger nodes depict “hub” proteins (hub score >0.8), with top-ranked hub protein in each case identified by a green node border.