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. 2020 Oct 5;18(12):3359–3370. doi: 10.1111/jth.15095

FIGURE 5.

FIGURE 5

JAK2V617F‐mutant cardiac endothelial cells (ECs) display a vasculopathy phenotype. A, Representative flow cytometry plots (left) and quantitative analysis (right) showing increased apoptosis in cultured JAK2V617F ECs (black bar) compared to wild‐type ECs (gray bar). B, Wild‐type and JAK2V617F‐mutant cardiac ECs (8 × 104) were seeded in Matrigel matrix in 48‐well plate. Representative tube formation after a 4‐hour incubation is shown. Magnification: 10×. C, Quantification of tube formation was performed on images taken at 4× magnification by counting the number of nodes (or branch points), tubes, and loops in four non‐overlapping fields. Results are expressed as the mean ± standard error of the mean (n = 4). Data are from one of two independent experiments on two different pairs of cardiac ECs that gave similar results. D, Gene expression level of EGFL7 in wild‐type (gray) and JAK2V617F (black) cardiac ECs measured using real‐time quantitative polymerase chain reaction. Gene expression in VF ECs is shown as the fold change compared to the average wild‐type EC expression which was set as “1.” (n = 3 in each group) *P < .05