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. 2020 Sep 17;46(12):2561–2572. doi: 10.1111/jog.14495

Table 1.

The primer sequences for RT‐qPCR

Gene Primer sequence
hsa‐miR‐139‐5p F: 5′‐GCCTCTACAGTGCACGTGTCTC‐3′
R: 5′‐CGCTGTTCTCATCTGTCTCGC‐3′
rno‐miR‐139‐5p F: 5′‐ACACTCCAGCTGGGTCTACAGTGCAC‐3′
R: 5′‐TGGTGTCGTGGAGTCG‐3′
hsa‐PTEN F: 5′‐GCATGTATTCGGGTTAGG‐3′
R: 5′‐TGAATGAAACTGACAAGG‐3′
rno‐PTEN F: 5′‐ TGGGAGTAGACGGATGCGAA‐3′
R: 5′‐ AACCAATCCGTGCCTTGACA‐3′
rno‐MMP2 F: 5′‐CCTTGGGGCAGCCATAGAAA‐3′
R: 5′‐TGTTTGGCAGAAGTTGGGGT‐3′
hsa‐U6 F: 5′‐CTCGCTTCGGCAGCACA‐3′
R: 5′‐AACGCTTCACGAATTTGCGT‐3′
rno‐U6 F: 5′‐GCTTCGGCAGCACATATACTAAAAT‐3′
R: 5′‐CGCTTCACGAATTTGCGTGCAT‐3′
hsa‐GAPDH F: 5′‐TCTCCTCTGACTTCAACAGCGA‐3′
R: 5′‐GTCCACCACCCTGTTGCTGT‐3′
rno‐GAPDH F: 5′‐CATCAACGACCCCTTCATTG‐3′
R: 5′‐GAAGATGGTGATGGGTTTCC‐3′

GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; hsa, Homo sapiens; miR‐139‐5p, microRNA‐139‐5p; MMP, metalloproteinase‐2; PTEN, phosphatase and tensin homolog deleted on chromosome 10; rno, Rattus norvegicus.