Table 1.
Gene | Primer sequence |
---|---|
hsa‐miR‐139‐5p | F: 5′‐GCCTCTACAGTGCACGTGTCTC‐3′ |
R: 5′‐CGCTGTTCTCATCTGTCTCGC‐3′ | |
rno‐miR‐139‐5p | F: 5′‐ACACTCCAGCTGGGTCTACAGTGCAC‐3′ |
R: 5′‐TGGTGTCGTGGAGTCG‐3′ | |
hsa‐PTEN | F: 5′‐GCATGTATTCGGGTTAGG‐3′ |
R: 5′‐TGAATGAAACTGACAAGG‐3′ | |
rno‐PTEN | F: 5′‐ TGGGAGTAGACGGATGCGAA‐3′ |
R: 5′‐ AACCAATCCGTGCCTTGACA‐3′ | |
rno‐MMP2 | F: 5′‐CCTTGGGGCAGCCATAGAAA‐3′ |
R: 5′‐TGTTTGGCAGAAGTTGGGGT‐3′ | |
hsa‐U6 | F: 5′‐CTCGCTTCGGCAGCACA‐3′ |
R: 5′‐AACGCTTCACGAATTTGCGT‐3′ | |
rno‐U6 | F: 5′‐GCTTCGGCAGCACATATACTAAAAT‐3′ |
R: 5′‐CGCTTCACGAATTTGCGTGCAT‐3′ | |
hsa‐GAPDH | F: 5′‐TCTCCTCTGACTTCAACAGCGA‐3′ |
R: 5′‐GTCCACCACCCTGTTGCTGT‐3′ | |
rno‐GAPDH | F: 5′‐CATCAACGACCCCTTCATTG‐3′ |
R: 5′‐GAAGATGGTGATGGGTTTCC‐3′ |
GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; hsa, Homo sapiens; miR‐139‐5p, microRNA‐139‐5p; MMP, metalloproteinase‐2; PTEN, phosphatase and tensin homolog deleted on chromosome 10; rno, Rattus norvegicus.