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a) Reaction catalyzed by BolR. b) Extracted ion chromatograms for m/z 130.9986, 808.1185, 191.0197, and 766.1079 corresponding to [M−H]− for 4, 5, 6, and 7 from negative ion mode UHPLC‐ESI‐Q‐TOF MS analyses of the BolR‐catalyzed condensation of oxaloacetate with acetyl‐CoA (top) and a negative control reaction containing BolR inactivated by boiling for 15 min prior to addition (bottom). The measured m/z values for citrate and co‐enzyme A were 191.0195 and 766.1076, respectively.
