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. 2020 Oct 19;59(52):23659–23667. doi: 10.1002/anie.202006260

Figure 6.

Figure 6

Ligation‐conjugation reactions; Additive‐free ligation conditions: 5 mM wrt selenol monomer (2.5 mM wrt diselenide dimer), and Cys‐peptide, 1.05 equiv. selenoester; Conjugation protocol A; 2.5 mM peptide (wrt selenol monomer), 50 equiv. TCEP, 2 equiv. TEMPO trap, 4 equiv. Mn(OAc)3, 50 °C, 1 hour; Protocol C; 2.5 mM peptide (wrt selenol monomer), 50 equiv. TCEP, 5 equiv. TEMPO trap, 37 °C, 4 hours; Protocol D; 2.5 mM peptide, 50 equiv. TCEP, 2 equiv. TEMPO trap, 10 equiv. Mn(OAc)3, 50 °C, 4 hours; [a] Overall yield over 2 steps.