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Left: Map of pBR322, showing the unique restriction sites inside both antibiotic resistance genes. Right: Generic map of a typical (empty) expression vector, having an origin of replication (for replication in vivo), a selective marker (Ampicillin resistance in this instance), and the T7 promoter and terminator flanking a His‐tag (to allow purification of the insert) and the multiple cloning site, which contains a large number of unique restriction sites (not shown) for easy cloning.
