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. 2020 Oct 26;59(51):23145–23153. doi: 10.1002/anie.202009007

Figure 6.

Figure 6

In vitro reconstitution of chain release by the PBS domain. a) Condensation of DHAP and the NAC thioester of 3‐ketooctanoate by the purified recombinant GdsF ACP‐PBS di‐domain and dephosphorylation of the resulting phosphorylated butenolide (7) using shrimp alkaline phosphatase. b) Extracted‐ion chromatograms at m/z=235.0925 (corresponding to [M+Na]+ for butenolide 8) from UHPLC‐ESI‐Q‐TOF‐MS comparisons of the enzymatic reaction cascade and a synthetic standard of 8. The ACP‐PBS di‐domain was omitted from the control reaction. c) Comparison of MS/MS spectra for the synthetic standard of butenolide 8 ([M+H]+=213.11) and the product of the enzymatic reaction cascade.