Table 1.
Melting temperatures T m for the Q–D hybrids without and with addition of 1 equivalent of PIQ in 100 mm NaCl, 20 mm sodium phosphate buffer, pH 7.[a]
|
Q–D hybrid |
T m (duplex) [°C] |
T m (quadruplex) [°C] |
||
|---|---|---|---|---|
|
|
w/o PIQ[b] |
with 1 equiv PIQ[b] |
w/o PIQ[c] |
with 1 equiv PIQ[c] |
|
Myc‐dup3 |
63.6±0.6 |
70.8±0.3 |
44.7±1.8 |
68.2±1.4 |
|
Myc‐dup5 |
67.8±0.6 |
73.5±1.0 |
53.2±0.6 |
69.7±1.2 |
|
Myc3l‐dup5 |
69.5±0.5 |
76.1±0.3 |
66.3±0.4 |
73.8±0.4 |
[a] Averages with standard deviation from three independent measurements except for the quadruplex melting of Myc3l‐dup5 with and without PIQ which was determined in duplicate. [b] T m data from UV melting experiments. [c] T m data from CD melting experiments.