Table 2.

ITC‐derived thermodynamic parameters for the binding of PIQ to the Q–D hybrids in the presence of 120 mm K⁺ at 40 °C.^[a]

Q–D hybrid	N	K a [m −1]	ΔH°fit [kcal mol−1][b]	ΔG° [kcal mol−1][c]	ΔH°es [kcal mol−1][b]	−TΔS° [kcal mol−1][c]
Myc‐dup3	1.2±0.1	(1.6±0.4)×107	−10.7±0.1	−10.3±0.2	−11.9±0.5	1.5±0.5
Myc‐dup3 [d]	1.1±0.2	(1.5±0.5)×107	−15.0±0.8	−10.6±0.2	−14.8±0.2	4.3±0.3
Myc‐dup5 [e]	n.d.	n.d.	n.d.	n.d.	−6.7±0.3	n.d.
Myc3l‐dup5	1.5±0.1	(1.6±0.2)×107	−8.2±0.1	−10.3±0.1	−7.3±0.2	−3.0±0.2

[a] Average values with standard deviations from three independent measurements; only values for the high‐affinity binding are given. [b] ΔH°_fit and ΔH°_es denote standard molar enthalpy changes determined from curve fitting and from an excess‐site method, respectively. [c] From ΔG°=−RTlnK _a and −TΔS°=ΔG°−ΔH°_es. [d] At 50 °C. [e] Isotherm could not be reliably fitted with a two‐site model.