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. 2020 Oct 11;104(4):1117–1128. doi: 10.1111/tpj.14989

Figure 3.

Figure 3

SrCYP92B14 catalyses oxidation of (+)‐sesamin with a catalytic outcome distinct from that of SiCYP92B14.

(a) RNA‐Seq analysis of SrCYP92B14 during Sesamum radiatum seed development. The expression profiles of SrCYP92B14, SrCYP81Q2, SrCPR1 and SrCYP98A20‐like during S. radiatum seed development were analysed by RNA‐Seq. FPKM; fragments per kilobase megareads. DAP, days after pollination.

(b) Phylogenetic analysis of SrCYP92B14 by the neighbour‐joining method using MEGA7. Bootstrap values (1000 replicates) are shown next to the branches. Scale bar represents the rate of substitutions/site.

(c) SrCYP92B14 oxidised (+)‐sesamin primarily to (+)‐sesaminol. Yeast cells expressing SrCYP92B14 together with SiCPR1 were subjected to bioassays using (+)‐sesamin as a substrate. Yeast cells harbouring the empty vector pYE22m were used as negative controls. Bioassay products after 48 h incubation were analysed on high‐performance liquid chromatography (HPLC). Numbers next to peaks indicate lignans corresponding to those in Figure 1c. 1: (+)‐Sesamin; 4: (+)‐sesamolin; 6: (+)‐sesaminol. Asterisks indicate (+)‐2‐episesaminol produced regardless of the expressed CYPs in the bioassays.