Table 2.
Molar ratios of (+)‐sesamolin and (+)‐sesaminol produced through paths a, b and c by CYP92B14 proteins using 2H‐labelled (+)‐sesamin as a substrate
| CYP | Ratio (%) | |||
|---|---|---|---|---|
| sesaminol | sesamolin | b/c | ||
| Direct oxidation (a) | ORA (b) | ORA (c) | ||
| SrCYP92B14 WT | 71 | 29 | trace | – |
| SiCYP92B14 WT | 7 | 11 | 82 | 13 |
| SiCYP92B14_Block190 | 11 | 15 | 74 | 20 |
| SiCYP92B14_A313S/S317A/T318V | 16 | 28 | 56 | 50 |
| SiCYP92B14_T121V | 4 | 24 | 72 | 33 |
| SiCYP92B14_T121V/A313S/S317A/T318V | 8 | 41 | 51 | 80 |
| SrCYP92B14_V120T | 87 | 13 | trace | – |
ORA, Oxidative rearrangement of α‐oxy‐substituted aryl groups.