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. 2020 Oct 27;38(12):1578–1593. doi: 10.1002/stem.3287

FIGURE 3.

FIGURE 3

FBLN5 is required for myelination of Schwann cells. A, A diagram of zebrafish larvae identifying the simplified peripheral nervous system, consisting of the spinal cord tracts, lateral lines, and the spinal motor nerves. A, anterior; P, posterior. B, Lateral view images of the whole‐mounted Tg(claudin K:gal4‐vp16;uas:egfp) zebrafish injected with control‐, fbln5‐MOs, or fbln5 mRNA after DAPI staining at 4 dpf. PLLn (green), posterior lateral line; A, anterior; P, posterior. OE, overexpression. Scale bars, 20 μm. C, Quantification of cell numbers counted within the PLLn of (B) images. Statistical significance was determined using one‐way ANOVA followed by Dunnett's post hoc test (*P < .05, **P < .005). D, Lateral view images of the Tg(claudin K:gal4‐vp16;uas:egfp) zebrafish injected with control‐MOs or fbln5‐MOs at 5 dpf. The images within the rectangles are magnified in the bottom panels. SCT, spinal cord tracts; PLLn, posterior lateral line; A, anterior; P, posterior. Scale bars, 100 μm. E, Quantification of the relative intensity of PLLn in equivalent fields of view in the images of (D). Statistical significance was determined using the unpaired Student's t‐test with Welch's correction (***P < .001). The data are shown as the mean ± SD of three independent experiments with ≥20 embryos per condition (C and E)