FIGURE 4.

FBLN5 regulates Integrin‐mediated actin dynamics in Schwann cells. A, Images of mouse primary Schwann cells double immunostained with Sox10 and F‐actin antibodies after treatment with recombinant FBLN5 alone or with an integrin inhibitor (Integrin Ihb). PBS‐treated Schwann cells were used as controls. Arrowheads and arrows indicate lamellipodia and filopodia, respectively. Scale bars, 20 μm. B‐D, Quantification of axial cell length in (B), lamellipodia number/cell in (C), and lamellipodia length/cell in (D) from data examined using the ImageJ software within equivalent fields of view in the images of (A). The data are shown as the mean ± SD of three independent experiments. Statistical significance was determined using one‐way ANOVA followed by Tukey's post hoc test (*P < .05, **P < .005; ns, nonsignificant)