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. 2020 Sep 16;46(12):2534–2546. doi: 10.1111/jog.14488

Figure 5.

Figure 5

miR‐149 disrupts the PTEN/Akt signaling pathway by targeting RUNX2. (a) TargetScan, StarBase, miRDB and miRTarBase predicted possible targeting mRNAs of miR‐149. (b) StarBase predicted the targeted binding sequence of miR‐149 and RUNX2. (c) Luciferase activity experiment verified the binding relationship between miR‐149 and RUNX2. (d) The mRNA expression of RUNX2 in placental chorionic tissues of 21 RM patients and 13 controls was detected by RT‐qPCR. (e) Pearson's correlation test analyzed the relationship between miR‐149 and RUNX2 in RM patients. (f and g). The mRNA and protein expression of RUNX2 in HTR‐8 and BEWO cells determined by RT‐qPCR and western blot analysis. (h) The protein expression of PTEN and Akt1 as well as the extent of Akt1 phosphorylation determined by western blot analysis. Comparisons between the two groups were performed using an unpaired t test (panel d). Two‐way ANOVA (panel c and h) or one‐way ANOVA (panel f and g) was adopted for comparisons among multiple groups, followed by Tukey's multiple comparisons test.