Table 2.
Targets | Prime sequences (5′–3′) |
---|---|
miR‐149 | Forward: CUUCGGAUCAGUGAAUGCUTT |
Reverse: AGCAUUCACUGAUCCGAAGTT | |
U6 | Forward: CTCGCTTCGGCAGCACA |
Reverse: AACGCTTCACGAATTTGCGT | |
RUNX2 | Forward: GCGUCUGUGUGAUUUGUAUTT |
Reverse: AUACAAAUCACACAGACGCTT | |
GAPDH | Forward: GCACCAGGCAGTGTAGTTAGC |
Reverse: GTGCAGGGTCCGAGGT |
Abbreviations: RT‐qPCR, reverse transcription quantitative polymerase chain reaction; miR‐149, microRNA‐149; RUNX2, runt‐related transcription factor 2; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase.