Figure 3.

Experimental protocol for the growth and expression of Aha‐labeled target proteins in MDS15 and MDS15A host strains. The addition of 3.5 g/L yeast extract to the medium leads to OD₆₀₀≈4.5 after about 8 h at 37 °C. After 3 h, 0.8 mM sodium azide and 0.8 mM pantothenic acid were fed to the cultures every 75 min, for a total of three times. After 8 h, 0.5 mM IPTG was added for induction of target protein expression, and the growth was allowed to continue at 28 °C for about 25 h. In the figure, the experimental growth rate diagram of MDS15A is shown.