Skip to main content
. 2020 Dec 21;4(2):e202000865. doi: 10.26508/lsa.202000865

Figure 7. ISRIB does not inhibit cytokines expression in LPS activated DCs and Macrophages.

Figure 7.

(A) IFN-β and IL-6 secretion was measured by Legendplex in WT and PERKΔK Flt3-L BMDCs stimulated with LPS during 4 h. (B) Protein synthesis was measured by puromycilation and flow in MEFs treated with ISRIB and different the integrated stress response inducing drugs, thapsigargin (Tg), subtilase cytotoxin (SubAB), and sodium arsenite for indicated times. (C, D) IFN-β, IL-6, IL-10, and TNF secretion was measured by Legendplex in (C) Flt3-L BMDCs and (D) M-CSF BMDM. Data are mean ± SD (n = 3). Statistical analysis was performed using Wilcoxon test (*P < 0.05 and **P < 0.01).