Figure S5. Steady-state DCs display eIF4F dependence for their protein synthesis.

(A) Monitoring of XBP1s mRNA splicing, ATF6, ATF4, and CHOP mRNA expression by qRT-PCR during Flt3-L BMDCs differentiation and in MEFs. (B, C) Protein synthesis activity upon ROC1 (B) or 4EGI (C) treatment of WT (left) and PERKΔK (right) Flt3-L BMDCs during time. DC do not display a chronic integrated stress response, nor become eIF4G-independent for their translation, as suggested for long term exposure to thapsigargin (Guan et al, 2017). (A, B, C) Statistical analysis was performed using Dunnett’s multiple comparison (A) and Mann–Whitney test (B, C).