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WT (a) and PPT1 cells (b–f) after 21 days of nitrogen-phosphorus-starvation with 10 mM acetate grown under alternating light/dark regime. a WT cells for comparison. b PTT1 cells showing a ruptured cell wall. c PPT1 cells with a single PHB granule. d PPT1 cells with multiple granules. e Fluorescence microscopic picture of PPT1 cells; PHB granules are visualized as red inclusions after staining with Nile red. f Overview of multiple PPT1 cells
