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mRNA expression of EMT and CSC markers in 5 days old HNSCC tumor spheroids ± CAFs relative to cells cultured in 2D. Relative levels of analyzed genes were calculated using the 2 − ΔΔCt method, and GAPDH and β-actin were used as internal standards. Data are normalized to cells cultured in 2D; n = 3 experiments, *p < 0.05 according to one-way ANOVA with Bonferroni adjustment
