Figure 2. Arouser is involved in resistance to starvation.

(A, C) Western blot analysis of endogenous (A) and overexpressed (C) Arouser in fed and 4–24 h starved larvae. (B) Analysis of aru mRNA levels in fed and starved larvae. Bar chart shows means ± s.d. Statistical significance was determined using two-tailed t test. (D, E) Survival of a 100 wild-type (w¹¹¹⁸) and aru mutant males fed on 5% sucrose (D) or water only (E). (F) Proportion of pupae form when well-fed second instar larvae are transferred onto water pads. (G) Survival on water only of the Arouser rescue line (rescue) compare with the corresponding mutant aru^8-128. (H) Western blot validation of the rescue line compared with wild-type and aru^8-128 mutant in fed and 4 h starved larvae. (I) Relative aru gene expression using primers that recognise endogenous and overexpressed aru (CDS) and primers specific for endogenous arouser (aru-RD). Bar chart shows means ± s.d. Statistical significance was determined using one-way ANOVA and based on at least three independent biological replicates, *P < 0.05.