Figure 6. Feeding, activity and aggressiveness behaviours are affected in aru-deficient flies.

(A, B) Relative quantification of the amount of food ingested by wild-type (wt) and aru mutant adult males fed on normal or high sugar diet. (A) Representative picture of flies after ingestion of coloured food are shown in (A). Bar chart shows means ± s.d. Statistical significance was determined using two-way ANOVA and based on the analysis of 20–40 individuals, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Comparison of the number of lunges per 20 min for aru mutants. (D) Comparison of the number of lunges per 20 min heterozygote and homozygote aru⁸⁸⁹⁶ mutants. (E) Comparison of the number of lunges per 20 min of flies silenced for aru specifically in the brain (Elav-G4) or fat body (ADH-G4). For the analysis of aggression phenotype (C, D, E), all the individual recordings are shown on charts. (F) Model of the balance between degradation and stabilisation of Arouser, and it’s implication in fly adaptation and survival during non-feeding states.