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. 2020 Dec 14;4(2):e202000965. doi: 10.26508/lsa.202000965

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© 2020 Jacomin et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — (A) Putative xLIR motifs in Arouser identified using the iLIR web-resource. (B) Colocalisation (arrowheads) of Arouser-GFP and mCherry-Atg8a in larval fat body cells. Scale bars: 10 μm. (C) Co-immunoprecipitation between endogenous Arouser and Atg8a in adult flies. (D) GST pull-down assay between GST-tagged Atg8a-WT or -LIR docking site mutant (K48A and Y49A), and radiolabelled myc-Arouser. (E, F) Western blot analysis of endogenous Arouser protein level in fed Atg8a (E) and Atg7 (F) mutant larvae.