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Quality Control Steps in Evaluating the Purity of Synthesized SPIONs and Isolated Lysosomes
(A and B) TEM images of synthesized SPIONs (scale bar in [A], 100 nm; scale bar in [B], 10 nm).
(C and D) TEM images and zoom (corresponding white square) of HeLa cells incubated with DMSA-SPIONs: (C) Pulse, 15 min; Chase, 1 h and (D) Pulse, 15 min; Chase, 4 h.
(E and F) Magnetically purified lysosomal pellet (E) and TEM image of the magnetically purified lysosomal pellet (F).
