Figure 1.

Generation of a Single-Cell Lung Suspension

(A) Mouse is sprayed with 70% Ethanol.

(B) Skin is removed and chest walls exposed.

(C) Removal of the chest walls to access the lobes of the lung.

(D) Lobes are removed.

(E) GentleMACS C Tube with the lung in the dissociation solution.

(F) Manual dissociation of the mouse lung in a 6 well-plate.

(G) Lungs are minced with scissors.

(H) Minced lungs.

(L) The lung is transferred to a 70uM cell strainer, using a wide-bore pipette tip.

(M) Lungs are grinded against the mesh of the cell strainer.