Reagent | Final Concentration (mM or μM) | Amount |
---|---|---|
PBS 1× | n/a | 47.75 mL |
FBS | 1% (v/v) | 0.5 mL |
EDTA | 5 mM | 0.5 mL |
Hepes | 25 mM | 1.25 mL |
Total | n/a | 50 mL |
Reagent | Final Concentration (mM or μM) | Amount |
---|---|---|
PBS 1× | n/a | 47.75 mL |
FBS | 1% (v/v) | 0.5 mL |
EDTA | 5 mM | 0.5 mL |
Hepes | 25 mM | 1.25 mL |
Total | n/a | 50 mL |