Figure 4.

Effect of digestion on coagulant activity of human milk (HM). HM (1% final concentration; vol/vol) was incubated in simulated gastric fluid (SGF) for 1 hour at 37°C at pH from 1 to 7 (A) and in simulated intestinal fluid (SIF) for 1 hour at 37°C with increasing concentrations of pancreatic enzymes (pancreatin) (B). After incubation of HM with SGF or SIF, samples were ultracentrifuged. Supernatant was removed, and pellets containing TF-exposing EVs were resuspended in the original volume to perform the clotting assay. **P < .01, ***P < .001.