Figure 5.

Flow cytometry to identify progeny of GFP- or YFP-transduced CD34⁺cells transplanted via either IB or IV routes, respectively. Fluorescence-activated cell sorting plots showing: gating by forward (FSS) and side scatter (SSC) to identify cell types (A), separation of GFP⁺ and YFP⁺ granulocytes from a control RM previously transplanted IV with CD34⁺ cells transduced with equal numbers of both vectors (B), and proportions of circulating granulocytes deriving from each graft (IV and OIB) in 3 RMs at 6, 9, and 12 days posttransplantation (C).