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The Processing of Mouse Back Skin
(A) The HBSS is turbid due to the presence of IMQ cream on the skin.
(B) After washing the skin rigorously for three times, the HBSS is transparent.
(C) 1 cm2-area of back skin is cut into four triangular pieces for subsequent digestion with Dispase II.
(D) Back-skin tissue fragments after mechanical disruption and before enzymatic digestion.
(E) Back-skin tissue suspension after 30 min of enzymatic digestion and pipetting.
