H20: |
4titude 96-well fully skirted PCR plate (FrameStar, 4titude, Cat# 4ti-0960/C) containing 50–180 μL of RNase-/DNase-free water (Life Technologies, #10977035) per well. This plate might be frozen and used several times |
cDNA: |
cDNA+amplicon mix 384-well plate from point 45, containing 12.5 μL amplified cDNA+amplicon mix after PCR |
Genotyping_stock |
4 empty 384 well plates (FrameStar, 4titude, Cat# 4ti-0384/C) |
Pool: |
96-well PCR plate (AB-0900; Thermo Scientific) on the top of a Corning 96-well plate tissue culture flat bottom PCR plate (SLS, Cat# 3595) |
Tipbox [1-4]: |
4 boxes of P50 tips, sterile, with barrier (Beckman Coulter, Cat# A21586) |