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. 2020 Oct 21;1(3):100125. doi: 10.1016/j.xpro.2020.100125
Genotyping-PCR1 Master Mix 1 Cell 110 Cells∗ (1 Quadrant) Storage Cat. No. Company
KAPA2G Robust HS Ready Mix 3.125 μL 343.75 μL −20°C KK5702 Sigma (Roche)
Primer pair 1 (F+R; 20 μM) 0.375 μL 41.25 μL −20°C Custom desalted oligos; resuspend in RNase-free TE Invitrogen
Primer pair 2 (F+R; 20 μM) 0.375 μL 41.25 μL −20°C
Primer pair 3 (F+R; 20 μM) 0.375 μL 41.25 μL −20°C
Water (DNase-/RNase-free) 0.5 μL 55 μL Room temp 10977035 Life Technologies
TOTAL 4.75 μL 522.5 μL