Genotyping-PCR1 Master Mix | 1 Cell | 110 Cells∗ (1 Quadrant) | Storage | Cat. No. | Company |
---|---|---|---|---|---|
KAPA2G Robust HS Ready Mix | 3.125 μL | 343.75 μL | −20°C | KK5702 | Sigma (Roche) |
Primer pair 1 (F+R; 20 μM) | 0.375 μL | 41.25 μL | −20°C | Custom desalted oligos; resuspend in RNase-free TE | Invitrogen |
Primer pair 2 (F+R; 20 μM) | 0.375 μL | 41.25 μL | −20°C | ||
Primer pair 3 (F+R; 20 μM) | 0.375 μL | 41.25 μL | −20°C | ||
Water (DNase-/RNase-free) | 0.5 μL | 55 μL | Room temp | 10977035 | Life Technologies |
TOTAL | 4.75 μL | 522.5 μL |