| Temperature | Time | Cycles |
|---|---|---|
| 95°C | 3 min | 1 cycle |
| 95°C | 00:15 | 35 cycles |
| 60°C | 00:20 | |
| 72°C | 1 min | |
| 72°C | 5 min | 1 cycle |
| 4°C | HOLD | 1 cycle |
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