| Dilution Name | Transfer (μL) | Mix with (μL of RNase-free TE) | Concentration (Relative to Stock) |
|---|---|---|---|
| Stock (E1) | NA | NA | 1 |
| E2 | 5 μL of E1 | 45 μL of TE | 1:10 |
| E3 | 10 μL of E2 | 990 μL of TE | 1:1,000 |
| E4 | 25 μL of E3 | 975 μL of TE | 1:40,000 |
| E5∗ | 100 μL of E4 | 900 μL of TE | 1:400,000 |
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