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. 2021 Jan 1;17(1):339–352. doi: 10.7150/ijbs.51241

Figure 1.

Figure 1

IFN-γ induces autophagy of cervical cancer cells and promotes phagocytosis and activation of macrophage. (A) HeLa and SiHa cells were treated with recombinant human IFN-γ at a concentration of 10ng/mL for 48 hours. LC3B was measured by western blot. (B) The autophagy level of HeLa and SiHa cells after treatment with IFN-γ was detected by DAP autophagy kit and observed with fluorescence microscope. Yellow arrows represented autophagosomes. Multiple fields of view were randomly selected through fluorescence microscope observation, and then the number of autophagosomes was calculated. (C, D) HeLa and SiHa cells were pretreated with IFN-γ or not, and then labelled with CFSE, and further co-cultured with U937 cells for 2 hours. The phagocytosis of U937 cells to HeLa and SiHa cells was tested by FCM. (E, F) HeLa and SiHa cells were pretreated with IFN-γ or not, and then co-cultured with U937 cells for 48 hours, and the expression of CD86 and CD163 on U937 cells were measured by FCM. IFN-γ: recombinant human IFN-γ. The results were expressed as mean ± SEM. NS: not significant difference, ** means P<0.01, and **** means P<0.0001.