Figure 6.

IDO1 inhibits tumor growth and promotes macrophage phagocytosis in vivo. (A) TC-1 cells were transfected with mus IDO1 overexpression or negative control lentivirus. And the IDO1 expression was measured by FCM. (B) LC3B in TC-1 cells transfected with lentivirus was measured by western blot. (C-E) TC-1 cells transfected with IDO1 overexpression or negative control lentivirus were injected subcutaneously in mice. Tumor volume was detected every other day. (F) Strategy of gating macrophage in tumor tissue. (G) Phagocytosis of macrophage towards GFP⁺ tumor cells were analyzed by FCM. (H) CD80 and CD206 were detected in macrophage (I-K) TC-1 cells transfected with IFNG overexpression or negative control lentivirus were injected subcutaneously in mice. And mice were administrated with epacadostat or control solvent every day. Tumor volume was detected every three days. (L) CD86 expression was detected in macrophage. The results were expressed as mean ± SEM. Epa represents for epacadostat. NS: not significant difference, * means P<0.05, ** means P<0.01, and **** means P<0.0001.