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. 2021 Jan 1;17(1):247–258. doi: 10.7150/ijbs.53477

Figure 4.

Figure 4

HES1 activates Slug transcription. A) The schematic model showed the three putative binding elements (BEs) within Slug promoter region including BE1: 5′-AAACCCAGGTGCCTA-3′ (-1644 to -1635); BE2: 5′-ATTTGCACGCGGCCGC-3′ (-624 to -615); BE3: 5′-AGAGCGTGGA-3′ (-241 to -232). B) HES1-binding sites within Slug promoter was detected in MDA-MB-231 cells by ChIP assay. C) Dual luciferase reporter assay in HES1-knockdown and control MDA-MB-231 cells, with co-transfection of wild-type or mutant-type Slug locus including BE1-MUT, BE1-MUT and BE1-MUT+BE3-MUT. Data are presented as the mean ± SD of three independent experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.