Fig 5.

Human primary keratinocytes gain stemness after transient transfection with siEcad+HKP complex. (A) Human primary keratinocytes underwent dedifferentiation after siEcad+HKP treatment. The cells were stained for E-cadherin (green), Propidium Iodide (red), KRT5 (green), KRT19 (red), and KRT15 (red). N = 3-5 rats per condition over four experiments. Scale bar: 50 µm. (B) Western blotting demonstrated that, after siEcad+HKP treatment, human primary keratinocytes expressed higher levels of KRT5, KRT15, and KRT19, but lower levels of E-cadherin. N = 3-5 experiments. (C) RT-PCR results for siEcad+HKP-treated human primary keratinocytes. CDH1 mRNA expression was reduced after the treatment, and KRT5, KRT15, KRT19, CD34, ITGB1, and ITGA6 mRNA expression increased significantly. N = 3 experiments. Values present the means ± SD. *P<0.05 **P<0.01, **P<0.01. ***P < 0.001.