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. 2020 Dec 8;45(2):693–705. doi: 10.3892/or.2020.7888

Figure 6.

Figure 6.

Blnk and Foxo1 limit the activity of Bcr-Abl kinase in BCR-ABL1-transformed pro-B cells. (A) Western blotting analysis of p-Crkl was performed in WT, Blnk-KO3 and Foxo1-KO3 BCR-ABL1-transformed pro-B cells treated with imatinib (0, 6 and 9 µM) for 24 h, respectively. The p-Crkl levels were quantified by the ImageJ program and normalized to total Crkl. Actin was used as a loading control. (B) The transcription of BIM, TRAIL, CDKN1B, and GADD45α in WT, Blnk-KO3 and Foxo1-KO3 BCR-ABL1-transformed pro-B cells. Gene mRNA fold expression values were normalized to the GAPDH. (C) Effects of deletion of Blnk on p-Foxo1 and p-Akt were measured with phospho-specific antibodies in WT and Blnk-KO3 BCR-ABL1-transformed pro-B cells treated with imatinib (0 and 6 µM). Blots were next stripped and reprobed with antibodies against total-Foxo1, total-Akt. Phosphorylated protein levels were quantified by the ImageJ program and normalized to total form antibodies. Actin served as a loading control. Data are representative of three independent experiments. Error bars represent the mean ± SEM. *P<0.05 and **P<0.01. Blnk, B-cell linker; Foxo1, forkhead box protein O1; WT, wild-type; KO, knockout; p-phosphorylated.