Figure 6.

ORFV induces apoptosis in CRC cells. (A) LoVo cells were seeded at a density of 1×10⁶ cells/well in 6-well plates and exposed to ORFV (MOI =5) or PBS for 24 h. Flow cytometry detected apoptosis in CT26 cells by Annexin V-APC/7-ADD staining. (B) LoVo cells were exposed to ORFV (MOI =5) for 0, 12, 18, 24, 36 and 48 h. Total protein of cells was used to detect the expression of the cleaved PARP, cleaved caspase-9, cleaved caspase-3 and Smac by western blotting. (C) Apoptosis of tumor cells was assessed and quantified by immunohistochemistry (cleaved caspase-3). (D) Immunistochemical score. ***P<0.05. ORFV, Orf virus; CRC, colorectal cancer; MOI, multiplicity of infection.