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. 2020 Dec 2;45(2):493–500. doi: 10.3892/or.2020.7878

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Expression and characterization of RIT. The pET32a(+)/Bs/CUS plasmid was transformed into E. coli BL21 (DE3) cells. (A) Schematic structure of pET32a(+)/Bs/CUS. (B) Use of 12% SDS-PAGE under reduced conditions. M, protein marker; 1, E. coli BL21 (DE3) transformed with pET32a(+)/Bs/CUS induced by 1 mM IPTG; 2, Bs/CUS purified by Ni-NTA column. 3, 7D12-9G8; 4, CUS. (C and D) Western blot confirmed the expression of Bs/CUS and CUS by using the primary antibodies against CUS and His tag, respectively. 1, Bs/CUS; 2, CUS. (E) EGFR expression on different cell lines. (F and G) Affinity of Bs/CUS compared with that of rE/CUS by flow cytometry. MFI, mean fluorescence intensity. *P<0.05 vs. SW620.