Figure 3.

Polyphyllin VII intensifies DRP1-dependent mitochondrial fission. (A) A2780 and SKOV3 cells were treated with various concentrations of polyphyllin VII for 24 h. Mito-tracker red fluorescence was used to evaluate mitochondrial fission, indicating the mitochondria in terms of the percentage of debris. Scale bar, 70 µm. (B and D) Polyphyllin VII treatment reduced DRP1 translocation to the mitochondria. Mitochondria were extracted for western blot analysis. (C and E) Quantification of Fig. 3B and D. (F) Western blot analysis of p-DRP1 and p-AKT in A2780 and SKOV3 cells after polyphyllin VII treatment. Polyphyllin VII treatment increased DRP1 and AKT phosphorylation. (G and H) Quantification of Fig. 3F. (I) After A2780 and SKOV3 were treated with polyphyllin VII at different concentrations for 24 h, PP2A activity assay was performed. Results are shown as mean ± SD from three independent experiments performed in triplicate. *P<0.05, **P<0.01, ***P<0.001, compared with the control (0 µM).