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. 2020 Dec 2;45(2):513–522. doi: 10.3892/or.2020.7879

Figure 4.

Figure 4.

Polyphyllin VII regulates the PP2A/AKT/DRP1 signaling axis in A2780 and SKOV3 cells. (A and B) A2780 and SKOV3 cells were pretreated with LB-100 for 1 h, followed by treatment with polyphyllin VII (3 µM) for 24 h. Mitochondria were extracted for western blot analysis. Western blot analysis of DRP1 and cytochrome c in A2780 and SKOV3 cells after polyphyllin VII treatment. (C) A2780 cells were pretreated with LB-100 for 1 h, followed by treatment with polyphyllin VII (3 µM) for 24 h. Co-localization (orange) of mitochondria (red) with DRP1 (green) was examined by Echo-lab Revolve microscopy (scale bar, 70 µm). (D) A2780 and SKOV3 cells were pretreated with LB-100 for 1 h, followed by treatment with polyphyllin VII (3 µM) for 24 h. Western blot analysis of p-DRP1 and p-AKT in A2780 and SKOV3 cells. (E) PP2A phosphatase activity detection after treatment. *P<0.05, **P<0.01, ***P<0.001 vs. untreated control; #P<0.05 vs. polyphyllin VII (3 µM) group.