Gating Strategy and Representative Data for Flow Cytometry Analysis
(A) Samples with a low level of anti-HBs titers. Each panel from left to right corresponds to a sequential gating strategy. Frequencies of HBsAg-specific memory B cells in PBMCs are shown in the right most panel. FSC, forward scatter; SSC, side scatter (-A, area; -H, height; -W, width).
(B) Samples with a high level of anti-HBs titers and a high level of serum neutralizing activities. The percentages of IgG+ memory B cells that bind to HBsAg antigen (HBsAg-PE+ and HBsAg-APC+) are significantly increased.