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. 2020 Oct 16;1(3):100129. doi: 10.1016/j.xpro.2020.100129

Figure 1.

Figure 1

Gating Strategy and Representative Data for Flow Cytometry Analysis

(A) Samples with a low level of anti-HBs titers. Each panel from left to right corresponds to a sequential gating strategy. Frequencies of HBsAg-specific memory B cells in PBMCs are shown in the right most panel. FSC, forward scatter; SSC, side scatter (-A, area; -H, height; -W, width).

(B) Samples with a high level of anti-HBs titers and a high level of serum neutralizing activities. The percentages of IgG+ memory B cells that bind to HBsAg antigen (HBsAg-PE+ and HBsAg-APC+) are significantly increased.