| Reagent | Final Concentration | Volume (μL) |
|---|---|---|
| 10× PBS (Cat#70013032, Thermo Fisher Scientific) | 0.5× | 200 |
| 0.1 M DTT (Cat#707265ML, Thermo Fisher Scientific) | 10 mM | 400 |
| RNAsin Plus RNase inhibitor (40 U/μL) (Cat#N2615, Promega) |
3 U/μL | 300 |
| Nuclease-Free Water (Cat#129114, QIAGEN) | n/a | 3,100 |
| Total | n/a | 4,000 |
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