Reagent | Final Concentration | Volume (μL) |
---|---|---|
Random Primers (0.3 μg/μL) (Cat#48190011, Thermo Fisher Scientific) |
18.3 ng/μL | 47 |
10% IGEPAL® CA-630 in Water (Cat#I8896, Sigma-Aldrich) | 0.61% | 47 |
RNAsin Plus RNase Inhibitor (40 U/μL) (Cat#N2615, Promega) |
0.78 U/μL | 15 |
Nuclease-Free Water (Cat#129114, QIAGEN) | n/a | 661 |
Total | n/a | 770 |