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. 2020 Oct 16;1(3):100129. doi: 10.1016/j.xpro.2020.100129
Reagent Final Concentration Volume (μL)
Random Primers (0.3 μg/μL)
(Cat#48190011, Thermo Fisher Scientific)
18.3 ng/μL 47
10% IGEPAL® CA-630 in Water (Cat#I8896, Sigma-Aldrich) 0.61% 47
RNAsin Plus RNase Inhibitor (40 U/μL)
(Cat#N2615, Promega)
0.78 U/μL 15
Nuclease-Free Water (Cat#129114, QIAGEN) n/a 661
Total n/a 770